Phenylpyruvate aminotransferase
Webl-tyrosine:2-oxoglutarate aminotransferase. Recommended name. tyrosine transaminase. Synonyms. l-phenylalanine 2-oxoglutarate aminotransferase. l-tyrosine aminotransferase. l-tyrosine-2-oxoglutarate aminotransferase. ... phenylpyruvate transaminase. phenylpyruvic acid transaminase. WebThe recombinant protein expressed in Escherichia coli is a homodimer with a subunit molecular weight of 42,000, has one pyridoxal 5'-phosphate per subunit, and is highly active toward glutamine, methionine, aromatic amino acids, and corresponding keto acids, but has no preference for alanine and dicarboxylic amino acids.
Phenylpyruvate aminotransferase
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WebFeb 1, 2016 · Recent evidence implicates a phenylpyruvate aminotransferase using l-tyrosine as an amino group donor to form l-phenylalanine (Yoo et al. 2013). The present data indicate that, unlike this tyrosine:phenylpyruvate aminotransferase and the phenylalanine:hydroxyphenylpyruvate aminotransferase from Atropa belladonna (Ab … WebIsoenzyme 1 was purified and characterized from liver mitochondrial extracts of the four species. Both rat and mouse enzyme preparations catalysed transamination between a number of L-amino acids and glyoxylate, and with L-alanine as amino donor the effective amino acceptors were glyoxylate, phenylpyruvate and hydroxy-pyruvate.
Webphenylpyruvate aminotransferase (human GlnAT; Ref. 6), rat GlnAT (7), and aromatic amino acid aminotransferase from Pyrococcus horikoshii (phAroAT; Ref. 8) with sequence identi-ties of 41.8, 36.5, 34.2, and 32.4%, respectively. We expressed the gene in Escherichia coli, purified the product protein, and physicochemically characterized it (9). WebSubsequently, phenylpyruvate is transaminated to Phe by a phenylpyruvate-aminotransferase (Fischer and Jensen, 1987a). The phenylpyruvate pathway is utilized for Phe biosynthesis in microorganisms, and the arogenate pathway has been proposed to predominate in the biosynthesis of Phe in plants ( Maeda et al., 2010 ).
WebOct 15, 2003 · The following three-dimensional structures of three forms of glutamine:phenylpyruvate aminotransferase from Thermus thermophilus HB8 have been determined and represent the first x-ray analysis of the enzyme: the unliganded pyridoxal 5'-phosphate form at 1 ... Macromolecules Find similar proteins by: (by identity cutoff) 3D … WebDownload Table Enrichment of PPY-AT activity in cytosol. from publication: An alternative pathway contributes to phenylalanine biosynthesis in plants via a cytosolic tyrosine:phenylpyruvate ...
WebGlutamine Phenylpyruvate Aminotransferase. Thus, although GTK probably contributes to the bioactivation of toxic halogenated cysteine S-conjugates in rat kidney, other lyases …
Web387861138 - EP 2328405 A4 20120248 - PLANT GLUTAMINE PHENYLPYRUVATE TRANSAMINASE GENE AND TRANSGENIC PLANTS CARRYING SAME - [origin: WO2010025465A1] The invention relates to transgenic plants exhibiting enhanced growth rates, seed and fruit yields, and overall biomass yields, as well as methods for generating … teams nrwWebNov 25, 2013 · Phenylpyruvate may originate from prephenate in plastids by the action of arogenate dehydratases (ADTs) [251,252] or in the cytosol, requiring a cytosolic pool of prephenate supposedly formed by... spaceless 2023WebMay 25, 2024 · Another strategy is transamination of l-phenylalanine to phenylpyruvate by aminotransferase. Several groups have demonstrated the biosynthesis of 2-phenylethanol from l -phenylalanine with a high titer by transamination (Kim et al. 2014 ; Yin et al. 2015 ). space lift renovations llcWebPhenylpyruvate tautomerase catalyzes the enolization of phenylpyruvate and related molecules with stereospecific removal of the pro-R hydrogen 45 (Scheme 9.19).Two … space liability conventionWebThe following three-dimensional structures of three forms of glutamine:phenylpyruvate aminotransferase from Thermus thermophilus HB8 have been determined and represent the first x-ray analysis of the enzyme: the unliganded pyridoxal 5′-phosphate form at 1.9 Å resolution and two complexes with 3-phenylpropionate and α-keto-γ-methylthiobutyrate at … spacelift operationsWebAug 1, 2024 · In this route, individually fed phenylpyruvate was decarboxylated to the corresponding aldehyde in a reaction catalysed by KivD from Lactococcus lactis and subsequently reduced to 2-PE by an endogenous aldehyde reductase, and 2-PE was esterified by ATF1 using acetyl-CoA, obtaining about 500 and 300 mg L −1 2-PEA and 2 … spacelift ioWebThrough the transamination reactions catalyzed by AspC or TyrB, L-phenylalanine (L-Phe) can be produced from phenylpyruvate with aspartic acid as the amino donor. To … teams nspcc